Antibacterial and cytotoxic activities of wild mushroom Fomes fomentarius (L.) Fr., Polyporaceae

Abstract The antibacterial activity of cyclohexane, dichlormethane, methanol and aqueous extracts of tinder fungus Fomes fomentarius (L.) Fr (Polyporaceae) was tested against 9 bacterial strains ( Staphylococcus aureus, Staphylococcus epidermidis, Micrococcus luteus, Bacillus subtilis, Enterococcus feacalis, Escherichia coli , Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella abony ), as well as against 10 different clinical isolates and one reference strain of Helicobacter pylori . Minimal inhibitory concentrations (MICs) of all extracts against 9 bacterial strains were in the range of 125–250 μg/ml. Methanol and aqueous extracts showed significant activity against H. pylori with MIC values between 4–32 μg/ml. Also, cytotoxicity of tested extracts was significant. Aqueous extract was the most active one against HeLa cells with an IC 50 8.31 ± 1.18 μg/ml and N87 cells with IC 50 64.46 ± 3.13 μg/ml without any activity against normal MRC5 cell line (> 200 μg/ml).