Cupriavidus nantongensis sp. nov., a novel chlorpyrifos-degrading bacterium isolated from sludge.
2016
A Gram-stain-negative, aerobic, coccoid to small rod-shaped bacterium, designated X1T, was isolated from sludge collected from the vicinity of a pesticide manufacturer in Nantong, Jiangsu Province, China. Based on 16S rRNA gene sequence analysis, strain X1T belonged to the genus
Cupriavidus
, and was most closely related to
Cupriavidus taiwanensis
LMG 19424T (99.1 % 16S rRNA gene sequence similarity) and
Cupriavidus alkaliphilus
LMG 26294T (98.9 %). Strain X1T showed 16S rRNA gene sequence similarities of 97.2–98.2 % with other species of the genus
Cupriavidus
. The major cellular fatty acids of strain X1T were C16 : 0, C16 : 1ω7c and/or iso-C15 : 0 2-OH (summed feature 3), C18 : 1ω7c and C17 : 0 cyclo, and the major respiratory quinone was ubiquinone Q-8. The major polar lipids of strain X1T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, aminophospholipid, phospholipid and hydroxyphosphatidylethanolamine. The DNA G+C content was 66.6 mol%. The DNA–DNA relatedness values of strain X1T with the five reference strains
C. taiwanensis
LMG 19424T,
C. alkaliphilus
LMG 26294T,
Cupriavidus necator
LMG 8453T,
Cupriavidus gilardii
LMG 5886T and ‘
Cupriavidus yeoncheonense
' KCTC 42053 were lower than 70 %. The results obtained from phylogenetic analysis, phenotypic characterization and DNA–DNA hybridization indicated that strain X1T should be proposed to represent a novel species of the genus
Cupriavidus
, for which the name Cupriavidus nantongensis sp. nov. is proposed. The type strain is X1T (=KCTC 42909T=LMG 29218T).
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