Serum ABO Blood Typing by Surface Plasmon Resonance Technique

Aim of this work is to develop a surface plasmon resonance (SPR) technique for detection of anti-A and anti-B which are the antibodies specific to ABO blood group in serum based on solid phase immobilization of antigens on the surface. Synthetic antigens A and B were immobilized on the carboxymethyldextran (CMD) surface to measure the antibodies. The immobilized synthetic antigen surface were tested function by injection of monoclonal anti-A and anti-B. Total 20 plasma samples at 1:10 dilution were measured by SPR techniuqe and the result were concordant to standard agglutination technique. The cost of blood typing could be economized by high throughput and surface regeneration on SPR technique.