Glutamate excretion in Escherichia coli : dependency on the relA and spoT genotype
1995
Glutamate excretion due to amino acid starvation was investigated in stringent and relaxed strains of Escherichia coli. The observed excretion process is relA-dependent, carrier-mediated, and glutamate-specific. After induction, excretion was detected within less than 2 min and continued for more than 5h with a rate of 7-10 nmol (mg dry weight) -1 min -1 . Using carbonyl cyanide m-chlorophenylhydrazone or polymyxin B nonapeptide, together with valinomycin, it was shown that glutamate excretion is driven by the membrane potential.
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