Effect of microRNA-195 on the expression of CCND1 in glioma U251 cell

Objective To research the effect of microRNA-195 on the expression of CCND1 in glioma U251 cell. Methods MicroRNA-195 mimics, microRNA-195 inhibitors were transfected into human glioma U251 cell by lipofectamine 2000 transient transfection. The microRNA-195 mimics transfection group was M group, and the microRNA-195 inhibitors transfection group was group I(blank control group, K group). Real-time PCR was used to detect the content of microRNA-195 before and after transfection to ensure the transfection efficiency. The expression of CCND1 mRNA was detected by real-time PCR. The expression of CCND1 in glioma U251 cells was detected by western blotting. Results Real-time PCR results showed: the expression of CCND1 mRNA in group M was lower than that in group I (M:758.264

Keywords:

• Blot
• Lipofectamine
• Cyclin D1
• microRNA
• Chemistry
• Molecular biology
• Glioma
• Transfection
• u251 cell
• Messenger RNA

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