Semiautomated enzymic micro methods for blood glucose and lactic acid on a single filtrate.

Methods have been modified and adapted to permit enzymic automated micro analysis of glucose on 0.021 ml of blood, and of lactic acid on 0.0115 ml of blood. By means of a single 1:20 Somogyi filtrate with a final volume of 1–1.4 ml prepared from 0.1–0.15 ml blood, both determinations can be accomplished individually on the autoanalyzer. The range of concentrations of each method has been extended so that glucose may be determined between 5 and 200 mg100 ml (or higher depending on dilution) and lactic acid between 5 and 125 mg100 ml. The methods are sensitive, reproducible, and stable. The recoveries and the comparison studies with manual methods are well within the limits of clinical investigation procdures.