Effects on different caging systems on behavioural testing in mice

The light-dark box apparatus contains a black plexiglass compartment (21 x 38 x 35 cm) and a clear, brightly lit (350 lux) plexiglass compartment (18 x 38 x 35 cm), separated by a small door (5 x 5 cm). Variables for estimating anxiety-like behaviour were the percentage of time spent in the light and dark compartments (sec) and the total number of transitions between the two compartments (frequency) . The testing period was 10 min for each mouse.