Differences of Dried Amniotic Membrane Effect on Healing of Gastric Tissue Post Penetrating Trauma Reviewed from Collagen Thickness and Fibroblast Count

Dried amniotic membrane (AM) has been shown to have the potential to help and facilitate proper closure of gastric tissue defect post penetrating injury trauma. This study evaluated the difference of fibroblast count and collagen thickness in gastric tissue post penetrating injury using with and without dried AM as a biological dressing. A randomized experimental study on New Zealand rabbits treated to an artificial gastric penetrating injury. The samples were divided into two groups, control and treatment, without and with dried AM, respectively. Microscopic evaluation of the gastric tissue specimen was done using hematoxylin-eosin staining. Average fibroblast count in the control group was 70.90±10.26, and the group with dried AM was 91.00±10.82 (p = 0.000). The mean thickness of collagen in the control group was 3142.48±1271.29 µm, and the group with dried AM was 4703.71±1807.88 µm (p = 0.006). These results showed significant difference in fibroblast count and collagen density when gastric penetrating injury was treated with primary closure and dried AM. There is a significant difference in the number of fibroblasts and collagen thickness in the repair of gastric rupture using dried AM as biologic dressing compared to the control group.