CLONING AND SEQUENCING OF PREPROTHYROTROPIN-RELEASING HORMONE AND ITS RECEPTOR IN THE RAT TESTIS

Objective\ In order to investigate the expression regulation of thyrotrophin\|releasing hormone(TRH) and TRH receptor (TRH\|R) in rat testis, and their function in production and development. Methods\ Total RNA of rat testis was used as template. The oligonucleotide primers were designed from the sequence of rat hypothalamus prepro(pp)TRH and pituitary TRH\|R cDNA for Reverse transcription\|polymerase chain reaction (RT\|PCR). Specific fragments of ppTRH and TRH\|R cDNA were cloned and sequenced. Results\ DNA sequence analysis indicated that cDNA sequences of ppTRH and TRH\|R from rat testis were consistent with those of hypothalamus ppTRH and pituitary TRH\|R cDNA. Conclusions\ It is the first time to acquire the cDNA cloning of ppTRH and TRH\|R from rat testis by RT\|PCR.