The optimal Factors for the Mycelial Growth of Sparassis crispa

This study was carried out to obtain the basic data for artificial culture of Sparassis crispa. The optimal conditions for the mycelial growth were and pH 4, respectively. The mycelial density of S. crispa was most compact in the Hamada media, whereas colony diameter was prominent in the Hoppkins media. Carbon sources such as maltose, arabinose and mannitol were favorable for stimulating a mycelial growth of S. crispa. Glycine, one of nitrogen sources also appeared to be favorable to the mycelial growth. The optimum C/N ratio was about 20 : 1 in case that 1% glucose as carbon source was mixed with the basal medium. Fumaric acid or lactic acid as organic acid was most favorable to the mycelial growth.