Identification of a Strain of Mycoplasma ovipneumonia from the Lung of a Neumonia Sheep

One Mycoplasma isolate XJ-3f was isolated from the lung of diseased sheep with the respiratory symptoms.The strain was cultured in modified Hayflick Medium and the culture were infected intratracheal to 2 sheep of 80 days.Lobular catarrhal bronchopneumonia was established by postmortem examinations,28 d from infection,and Mycoplasma was re-isolated from the lungs.We designed a pair of primers to amplify a 700 bp fragment of 16S rRNA gene of all Mycoplasma.The genomic DNA of lung were extracted,amplified by the primers and cloned into the pMD18-T vector.33 reference strains were loaded down from GenBank,the result of sequence analysis indicated that the isolated strain,XJ-3f,exhibited 99.9 % homology with reference strain Y-98,81 % with reference strains Mccp、MmmLCMccMmc.The result of Western blot showed that they are all recognized by the serum as seem as Y-98.The results indicated that the isolated strain XJ-3f is belong to the Mycoplasma ovipneumonia.