Influence of radiation at different RF frequencies on Bovine Serum Albumin stability in the aspect of biosensor

Scans of electromagnetic field distribution for (125, 180 and 230 MHz) have been collected with the use of 3D EMC scanner and specially constructed experimental setup. The analysis of UV/Vis spectrum was used for testing influence of electromagnetic field on Bovine Serum Albumin and changes through 19 days. The protein beyond the wide application in pharmacokinetics and pharmacodynamics drug study, is also widely used in the biosensors construction. In the presented study, taking into account existing problems associated with biosensor receptor layer stability, the effect of the influence of electromagnetic field as a function of time on Bovine Serum Albumin (BSA) was investigated. Increase of absorption of BSA with time, related to conformational changes of protein was observed. The differences between irradiated and control samples have been noticed. The impact of electromagnetic field on protein showed tendency to BSA stabilization under 125, 180, 230 MHz exposition. Changes were the most notable for 230 MHz where as 125 MHz and 180 were small but significant.