ELISA (Enzyme Linked Immunosorbent Assay) kit for detecting porcine epidemic diarrhea virus antibody

The invention provides an ELISA (Enzyme Linked Immunosorbent Assay) kit for detecting a porcine epidemic diarrhea virus antibody, belonging to the technical field of biology. The kit is an ELISA plate stripe using purified recombinant porcine epidemic diarrhea virus N protein as an envelope antigen. RT-PCR (Reverse Transcription-Polymerase Chain Reaction) amplification is adopted for obtaining a porcine epidemic diarrhea virus N gene, the gene is directionally inserted into a pET-28a carrier to construct pET-28a-N recombinant plasmids, BL21 (DE3) competent cells is transformed, and finally a prokaryotic expression fungus pET-28a-N/BL21 is obtained. The prokaryotic expression fungus pET-28a-N/ BL21 is subjected to induced expression and purification through IPTG (Isopropyl Thiogalactoside) to obtain recombinant N protein. The kit is used for detecting the porcine epidemic diarrhea virus antibody. By testing, the kit has good specificity, sensibility and repeatability. The kit is accurate in detection, easy and convenient to operate and suitable for promotion in clinical application and provides a reliable means for rapid detection of porcine epidemic diarrhea.