PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL EFFECT OF ZIZYPHUS LOTUS METHANOLIC EXTRACT AGAINST ORAL PATHOGENIC BACTERIA

The aim of this in vitro study was to assess the antibacterial effect of the methanolic extract obtained from the roots of Zizyphus lotus (communally Sedra) collected in Mascara on bacteria responsible of oral pathologies, isolated from students aged from 20 to 28 years. Chemical qualitative analysis of this extract revealed the presence of polyphenols, flavonoids and tannins confirmed by a quantitative analysis which revealing 433,65μg EGA / mg dm of polyphenols, 247,87μg EQ/mg dm of flavonoids and 167.05 µg ECT/ mg dm of tannins. The microbiological analysis of samples collectedfrom the oral cavity (10 students) revealed four bacterial groups (streptococci, staphylococci, lactobacilli and enterobacteria). The methanolic extract of Zizyphus lotus tested on agar medium and microdilution method showed an important inhibition diameter of 40 mm against Serratia liquenfaciens and MIC of 6.25mg/ml respectively towards Lactobacillus sp. and Escherichia coli .