FORMATION OF SECONDARY PLASMODESMATA AND INTERCELLULAR PASSAGES BY MEANS OF ACHT

By artificially forcing cytoplasm and chromatin migration between different plant cells and through coculture, the isolation layer with uneven thickness was formed between tobacco and spinach cells. Then it was gradually absorbed, thinned, and eliminated finally, so that different plant cells were linked together with each other into chimaera. Under further co-culture condition, secondary half-plasmodesmata, secondary plasmodesmata and intercellular passages (cytomictic channels) were formed on one side or both sides of the walls of the adjoining cells; thus different plant cells were linked into symplastic cell mass. This provides the cell structural and cell physiological basis for further manipulating the intercellular migration of cytoplasm and chromatin. Various intercellular contacts and passages are described.