[HIV-1 infection up-regulating expression of interferon-stimulated gene 15 in cell lines].

To investigate whether HIV-1 infection affects expression of interferon-stimulated gene 15 (ISG15 ) and determine the antiviral effect of ISG15 in vitro,ISG15 expression at transcription and protein level and supernatant p24of HIV-1 was detected in various HIV-1infected or transfected cell lines,respectively.HIV-1 molecular clone pNL4-3was used to transfect 293T,TZM-bl and HeLa cells while HIV-1 pseudo-typed virus was used to infect Jurkat,MT-1 and THP-1 cells.After twenty-four hours post infection or transfection,cells were harvested for extraction of total RNAs and subsequently used in real time PCR for quantification of ISG15 transcriptional expression.After forty-eight hours post infection or transfection,cells were harvested for extraction of total proteins to detect ISG15 protein expression.A significant up-regulation of ISG15 at transcription level was observed in HIV-1 infected or transfected cell lines,particulaly in THP-1 and TZM-bl cells.Up-regulation of ISG15 protein was observed only in TZM-bl cell.Cotransfection of ISG15 and HIV-1 indicated that ISG15 inhibited production of HIV-1 progeny virus in a dose and time depend manner in 293T cell but not TZM-bl cell.These results revealed upregulating ISG15 expression in transcriptional level and potential antagonistic mechanism against ISG15 by HIV-1 infection,simultanelusly.