MECHANISM OF POLIOVIRUS MULTIPLICATION.

Abstract : There is considerable inhibition of virus multiplication if AMD is added to HEp 2 cell prior to or together with the virus. This is not ascribable to permeability hindrance of viral penetration. In order to determine the mechanism of this inhibition, infected and non infected cells, treated or not with AMD, were pulse labeled with P32 or uracyl -C14, at various periods during their multiplication in spinner culture. The purified macromolecules were resolved on sucrose gradient centrifugation. The results showed that there is decrease of both the double and single stranded poliovirus like RNA, in the various time periods analyzed. RNA polymerase, RNA dependent, has been detected in poliovirus infected HEp 2 cells. Efforts have been made to purify this enzyme, and to analyze the requirements of the enzyme system. It has been found that two types of cell membranes are able to incorporate AA-C14. Some properties of this protein synthesizing system have been studied. (Author)