Suspension culture ofNigella sativa

In suspension culture 91% free cells ofNigella sativa was obtained inWhite's medium supplemented with casein hydrolysate, inositol and adenine. Ploidy distribution pattern was similar in cell clumps of different sizes and free cells. Chromosomal irregularities were more in free cells. A number of globular embryoid were formed when casein hydrolysate, inositol and adenine were added in the medium after subsequent omission of auxin and coconut milk.