EFFECTS OF GLUTAMINE ON GROWTH AND METABOLISM OF CHINOOK SALMON EMBRYO (CHSE) CELLS

The difference has been known in glutamine metabolism between fish and mammalian. In order to optimize the medium and the culture process for CHSE cells, the effects of glutamine were investigated on the cell growth and metabolism. Glutamine-free medium-199 could support the growth of CHSE cells, as well as medium-199 with glutamine. The maximum cell density, 16.19×105 mL -1, was obtained at 288 h in the culture with 0.54 mmol L -1 glutamine. However, higher glutamine (c= 1.76～ 5.62 mmol L -1) inhibited the growth of CHSE cells. With the increase of initial glutamine from 0～5.62 mmol L -1, the total utilization of glucose and the production of lactate were almost not affected.But the yield coefficient of cell to glucose (Y n/glc) was reduced, and the specific rates of glucose consumption (Q glc) and lactate production (Q lac) increased. On the other hand, with the increase of c gln (0～3.33 mmol L -1), the glutamine consumption was increased,the yield coefficient of cell to glutamine (Y n/gln) and ammonia to glutamine (Y ammo/gln) dropped by 58% and 50%, respectively, and then kept constant with c gln up to 5.62 mmol L -1. Besides, alanine was consumed in the absence of glutamine, and the yield coefficient of alanine to glutamine (Y ala/gln) first increased with the increase of c gln from 0.54 mmol L -1 to 1.76 mmol L -1, then dropped with c gln up to 3.33 mmol L -1, and finally kept constant between 3.33 mmol L -1 and 5.62 mmol L -1 c gln. Fig 4, Tab 3, Ref 16