Study on the relationship between advanced glycation end products and cystanthionine-γ-lyase, hydrogen sulfide in the penis of diabetes mellitus erectile dysfunction rats

Objective Diabetic rats were treated with aminoguanidine, in order to investigate the relationship between advanced glycation end products (AGEs), cystanthionine-γ-lyase (CSE) and hydrogen sulfide (H2S) in diabetic rats penis. It was to confirm that AGEs impacts the erectile of the rats with diabetes mellitus erectile dysfunction through hydregen sulfide pathway. Methods The cleaning grade Wister rats of 6 weeks old, male, weighing about 180-220 g, a total of 60 rats, were randomly divided into group A (10 rats), group B(10 rats) and model group(40 rats). Group A and group B with free drinking and eating. The model group were given free drinking water, high fat and high-glucose diet for 1 month. In model group, 23 diabetic model rats were induced, which were randomly divided into group C of 12 rats and group D of 11 rats. Group A were continued to feed with free drinking and eating, group B were continued to normal diet and every litre drinking water was added 1 gram aminoguanidine; group C were continued to feed with high fat and high-glucose diet, free drinking water; group D were continued to high fat and high-glucose diet, every litre drinking water was added 1 gram aminoguanidine. After 6 weeks of feeding, each group rats were measured the intracavernous pressure and mean arterial pressure. The concentration of hydrogen sulfide in serum and penis tissue were measured. Meanwhile AGEs, receptor for advanced glycation end products (RAGEs), CSE in penile tissue were observed through immunohistochemical; RAGEs and CSE in penile tissue were measured by Western blotting. Results The difference of ICP/MAP and the concentration of hydrogen sulfide between the group A and the group B which were treated by aminoguanidine had no statistical significance (P=0.125); the difference of intracavernous pressure (ICP)/mean arterial pressure (MAP) between the group C and the group B was statistical significance, and the difference of contents of AGEs, RAGEs, CSE and H2S in the penis between the group C and the group B was statistical significance (P=0.001); the difference of ICP/MAP and contents of AGEs, RAGEs, CSE and H2S in the penis between the group A and the group C was statistical significance. Conclusion In diabetes mellitus rats, AGEs can affect the production of CSE and H2S through RAGEs, and then affect erectile function in the rats. Key words: Diabetes mellitus; Erectile dysfunction; Advanced glycation end products; Hydrogen sulfide; Cystanthionine-γ-lyase