The effect of two fixation methods (TAF and DESS) on morphometric parameters of Aphelenchoides ritzemabosi

Identification of nematode species by using conventional methods requires fixation of the isolated material and a suitable preparation for further analyses. Tentative identification using microscopic methods should also be performed prior to initiating molecular studies. In the literature, various methods are described for the preparation of nematodes from the genus Aphelenchoides for identification and microscopic studies. The most commonly used fixatives are formalin (Timm 1969; Szczygiel & Cid del Prado Vera 1981, Crozzoli et al . 2008, Khan et al . 2008), FAA (Wasilewska 1969; Vovlas et al . 2005, Khan et al . 2007) and TAF (Hooper 1958, Chizhov et al . 2006, Jagdale & Grewal 2006).