Influence of the culture media on the aspect and growth of oilseed rape fungal pathogens

While culturing fungal pathogens on artificial media, the phenotype, i.e. colony aspect, growth kinetics and in some case the pigment production can be used as discrimination criteria. Our aim was to enable the comparison between the growth kinetics of 8 fungal species, cultured simultaneously on 3 different media, and imaged under standardised conditions. Using a camera stand, taking pictures was quick and easy, producing homogeneous pictures, facilitating their comparison. The colony growth kinetics varied widely across the 8 species. The mycelium aspect and pigment production depended on the media and changed over time. Having standardised the imaging setup and grown species simultaneously allows proposing reference sets of pictures.