ANP Attenuates Intestinal Inflammation by Regulating STING Pathway

Atrial Natriuretic Peptide (ANP) showed anti-inflammatory effects in recent studies. However, the specific role of ANP in inflammatory bowel disease (IBD) remains unknown. The study was designed to investigate the expression and function of ANP in IBD and identify whether it regulated IBD by the stimulator of interferons genes (STING) pathway. The ANP and STING pathway levels were detected in IBD patients or dextran sulphate sodium (DSS)-induced colitis mice. We found that ANP treatment meaningfully attenuated DSS-induced colitis and inhibited the phosphorylation of the STING pathway in colon tissues. Besides, ANP repaired the intestinal mucosal barrier that damaged by colitis via the STING pathway. ANP may be a promising therapeutic option in IBD treatment. Funding Statement: This study is funded by National Natural Science Foundation of China [grant number 82070572, 81770554, 81772607, 81974383]. Declaration of Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Ethics Approval Statement: This study was authorized by Ethics Committee of Tongji Medical College, Huazhong University of Science and Technology, with the approval number [2020]IEC-J(333). All patients provided written informed agreement. All animal experiments were carried out following the principles of Declaration of Helsinki with the approval of the Animal Ethics Committee of Tongji Medical College. (No. S2382).