INTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES Antimicrobial activity of ethanolic, methanolic and hot aqueous extract of Punica granatum peel
2013
The main objective of the study is to evaluate anti bacterial activity of Punica granatum pericarp (peel) extracts (hot aqueous, methanolic and ethanolic) against E. coli and P. aeruginosa , using Zone of Inhibition (ZOI) and Minimum Inhibitory Concentration (MIC) method. Hot aqueous, methanolic and ethanolic extracts of P. granatum pericap showed an average inhibitory zone diameter of 21.5, 22.5 and 23mm respectively which indicates that et hanolic extract showed best result having ZOI greater than that of the standard antibiotic Tetracycline (20.1m m) against E. coli while hot aqueous, methanolic and ethanolic extra cts of P. granatum pericap showed an average inhibitory zone diameter of 21, 23.5 and 25.5mm respectively which indicates that ethanolic extract showed best r esult having ZOI greater than that of the standard antibiotic Te tracycline (22.5 mm) against P. aeruginosa . P. granatum peel has MIC of 0.312 µg/ml against E. coli and 0.421 µg/ml against P. aeruginosa . Data revealed that ethanolic extract of P. granatum peel has shown highest antimicrobial activity as c ompared to other extracts.
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