Effects of pioglitazone on the proliferation and TGF-β1 expression of rat peritoneal mesothelial cells cultured in high glucose concentration.

Objective To observe the effects of pioglitazone on the inhibition of cell proliferation and the expression of transforming growth factor-β1 (TGF-β1)in rat peritoneal mesothelial cells (RPMCs)cultured in high glucose concentration. Methods RPMCs were isolated from rat colic omentum and cultured in high glucose concentration(2.5% glucose). MTT method was processed to observe the effects of pioglitazone (5,10,20μmol/L)on the cell proliferation. ELISA analysis were performed to investigate the change of TGF-β1 concentration in the culture medium. Results High glucose concentration of 2.5% could significantly inhibit cell proliferation as well as induce the expression of TGF-β1 in RPMC(P 0.01),which could be partially reversed by pioglitazone of 5～20 μmol/L(P 0.01). Conclusion Pioglitazone could reverse both the cell proliferation inhibition and fibrosis cytokine TGF-β1 induction by high glucose in RPMC,and repair the cell damage induced by high glucose consequently.