Comparison of values of FQ-PCR and immunoblotting in determination of human cytomegalo virus.

Aim To compare the value of fluorescnet quantitative polymerase chain reaction(FQ-PCR)in detection of human cytomegalo virus(HCMV)infection with immunoblotting(IBT).Methods Urine and blood samples were collected from 84 patients with suspected HCMV infection.Urine samples were used for detecting HCMV DNA by FQ-PCR,Blood samples were used for simultaneously detecting HCMV IgM antibody by IBT assay.Results The positive rate of HCMV DNA was 57.14%,and that of HCMV IgM was 35.71% among 42 children,FQ-PCR was superior to IBT(χ2=3.88,P0.05),showing significant difference;The positive rate of HCMV DNA was 14.29%,and that of HCMV IgM was 71.43% among 42 adults,IBT was superior to FQ-PCR(χ2=28,P0.005),showing significant difference.;The positive rate of HCMV DNA was 38.10%,and that of HCMV IgM was 48.81% among all 84 patients,the difference of the two methods for diagnosis of HCMV infection was not significant(χ2=1.96,P 0.1).Conclusions FQ-PCR is a sensitive assay for diagnosis of HCMV infection in children;IBT can be used as a diagnostic assay for active HCMV infection in the adult;and IBT is a good assay for the general investigation.