POLYPLEX SYNTHESIS BY ''MICROFLUIDIC DRIFTING'' BASED THREE-DIMENSIONAL HYDRODYNAMIC FOCUSING METHOD

We synthesized polymer-DNA nanocomplexes (polyplexes) using a “microfluidic drifting” based three-dimensional (3D) hydrodynamic focusing in a single-layered, microfluidic device. This synthesis method requires no additional chemical treatment steps or any post processing. Compared with the conventional bulk mixing method [1,2], the polyplexes prepared by our “microfluidic drifting” 3D focusing method [3,4] showed smaller size, slower aggregation rate and higher transfection efficiency. This “microfluidic drifting” 3D focusing method provides a simple, fast, and repeatable method to synthesize high-quality polyplexes, which can be used in nucleic acid therapeutics to reduce the cost and complexity..