Properties of the CMCase produced by Pseudomonas sp. YD-15

A bacterium having CMCase activity was isolated form soil and identifed as a Pseudomonas sp YD-15. The optimum conditions for the production of CMCase were avicel 1.2%, yeast extract 0.5%, 0.06%, 0.2%, 0.15%, pH 8.0, and 60 hours cultivation. The CMCase was purified 15.2 folds with 14ft yield through ammonium sulfate precipitation, DEAE-sepharose column chromatography and sephadex G-100 gel filtration chromatography. The optimum pH and temperature for the enzyme activity were 6.0 and , respectively. The enzyme was stable between pH 5.0 and 8.0, below . The molecular weight was calculated about 100,000 by SDS-polyacrylamide gel electrophoresis. value for CMC used as a substrate was 40 mg/ml.