Genetic Diversity and Relationship Analysis of Asarum Germplasm Resources Based on ISSR and SRAP Markers

To analize the genetic diversity and relationship of Asarum germplasm resources,sixty-one Asarum germplasm were tested using ISSR and SRAP Markers. The mean bands amplified per primer by ISSR and SRAP were 8. 35 and 7. 85 respectively. The percentage of polymorphic bands was 86. 3% and 86. 0% for ISSR and SRAP respectively. The ISSR has higher polymorphism than SRAP. The cluster of accessions was associated with collection regions. Comparing to ISSR,SRAP marker had the advantage in reflecting the difference of genome. Accessions of Asarum heterotropoides Fr. var. mandshuricum( Maxim) Kitag. and Asarum sieboldii Miq. var. seoulense Nakai from same region clustered into the same sub-clade,which indicats they have close genetic relationship. Taxonomy based on molecular markers was not consistent with that based on botanic characteristics,because notr all A. heterotropoides collections were clustered into one major clade,similar situation was detected for A. sieboldii collections too.