Method for separating and purifying Aspergillus oryzae transformant

2013 
The invention relates to a method for separating and purifying an Aspergillus oryzae transformant and a method for producing a target protein by adopting Aspergillus oryzae. In the method for separating and purifying the Aspergillus oryzae transformant, one process of carrying out mononuclear separation by adopting an inositol-containing culture medium is added before the transformant is purified by utilizing the traditional monospore isolation, so that the transformant separating and purifying efficiency is greatly improved, and manpower cost and time cost are greatly reduced; besides, by improving the transformant separating and purifying efficiency, the efficiency of expressing the target protein by adopting the Aspergillus oryzae is also greatly improved.
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