Effects of B17 vitamin onsphingosine-1 phosphate and oxidative/nitrosative stress in the experimental model ofheart ischemia/reperfusion. -

Demirhan I, Kurutas EB, Bakaris S, Ozturk U, Oner E. Effects of B17 vitamin on sphingosine-1 phosphate and oxidative/nitrosative stress in the experimental model of heart ischemia/reperfusion. Adv Lab Med Int. 2018; 8: 18-36. ABSTRACT - Ischemia refers to the reduction or cessation of blood flow which results in tissue damage and causes insufficient oxygen and nutrition to the tissues. For the continuity of heart tissue, the early reperfusion of the ischemic area is essential. However; the reperfusion itself, too results in the death of the heart cells which is called reperfusion injury. After the cardiac ischemia, the oxidative stress caused by the reperfusion leads to serious functional and structural damage. Free oxygen radicals are held responsible for this damage. Sphingosine 1- Phosphate (S1P), one of the secondary messengers in Sphingolipid structure and having a mission in intracellular signal transduction, has a quite important role in the growth, life and death of a cell and, no studies about vitamin B17 on cardivascular diseases are encountered. Therefore,we aimed to investigate the effects of B17 vitamin on the S1P and oxidative/nitrosative stress biomarkers in heart ischemia/reperfussion. In this study, for the first time, vitamin B17’s biochemical and histopathological effects on cardiac ischemia-reperfusion injury were investigated on 24 adult female rats. Rats were randomly divided into 3 groups: Control (n=8), Sham (n=8) and B17 therapy group (n=8). While 2 days before the experiment, 1 mL normal saline (0.9% NaCl/kg/day and 50 mg /kg/day) was started to apply to the ratsin Sham and B17 groups intraperitoneally once a day, nothing was implemented on the control group. Then; after the venture and surgical procedure applied to the all rats groups, 10 minutes ischemia and 10 minutes reperfusion of the heart was created. In pursuit of the reperfusion, again 1 Ml normal saline (single dose) and B17 (single dose) were applied to the rats of Sham and B17 groups while nothing was implemented on the control group. In the 0th minute of reperfusion, blood was taken from all groups and CK-MB value was measured. At the end of the experiment, all rats were sacrificed, and as the markers of oxidative/ nitrosative stress on heart tissues, the levels of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), malondialdehyde (MDA), nitric oxide (NO) and the nitrotirozin (3-NT) were measured. Also, S1P was measured by ELISA. At the end of the biochemical and histopathological examinations; when the Sham, Control and Therapy groups were compared, significant differences were found(p less than 0.05). In the Control, Sham and B17 groups, at the 0th minute of the reperfusion, the cardiac enzyme CK-MB level’s average peak values were 1.07 ng/ml, 0.98 ng/ml and 0.93 ng/ml respectively. However; a statically significant difference could not be found between the groups. (p more than 0.05). In comparison with the B17 theraphy group, the levels of MDA, NO AND 3-NT were high in Control and Sham groups while CAT, SOD and GPx activitiese were quite low. (p less than 0.05). In Control and Sham groups, statically significant differences were not determined in terms of the MDA,NO,3-NT, CAT,SOD and GPx levels. (p more than 0.05)(truncated) Full text availabe from Advance Laboratory Medicine Journal at https://drive.google.com/file/d/1yRNyfK8iBOUZVgUUU0jkbcG9IwsYxWLG/view