Reconstruction Of 3D Light Microscopic Images

Light-microscopic images of three-dimensional objects are distorted by defocused projections into the focus plane. A reconstruction procedure for three-dimensional light-microscopic images based on the linear system theory is described. Reconstruction is executed by inverse filtering of the image using an approximation of the three-dimen-sional optical transfer function of the microscope. The derivation and the features of this transfer function are out-lined. The reconstruction procedure permits to achieve a significant improvement of the image quality. The recon-struction result of a test object is shown.