巨噬细胞移动抑制因子－173G/C基因多态性与紫癜性肾炎的相关性

Objective To explore the correlation between -173G/C gene polymorphism of macrophage migration inhibitory factor (MIF) and Henoch-Schonlein purpura(HSP), Henoch-Schonlein purpura nephritis (HSPN) in children in Jiangxi Province. Methods One hundred and thirty-one ethnic Han children with HSP were enrolled, including 80 children with concurrent nephritis (HSPN group) and 51 children without nephritis (HSP without nephritis group). One hundred and five healthy children were used as the healthy control group. Germ-line DNA was extracted from peripheral blood by Promega blood genomic DNA kit. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used for genotyping the -173G/C polymorphism of MIF. Genotype distribution and allele frequencies were obtained by direct counting. Statistical analysis was performed by using SPSS 11.5 software. Allele and genotype distribution were compared by using the chi-square test. The relative risk of allele was described by odds ratios (OR) and 95% confidence intervals (95% CI). Results Three genotypes (GG, CC; CC) were detected in MIF-173 G/C. GG, GC genotypes were detected in HSP without nephritis and healthy control group. GG, GC and CC genotypes were detected in HSPN group. Mutant genotype (37.5%) and C allele frequency (20.0%) in HSPN group were significantly higher than those in healthy control group (20.0% and 10.0%, respectively) (X^2=6.964, 7.400, P(subscript a)＜0.01). Conclusions MIF-173G/C gene polymorphism may be associated with the development of HSPN, C allele may be a susceptible gene of HSPN.