Base Modified Primeroligomere for the multiplex RT-PCR

The invention relates to a method for amplification of nucleic acid sequences by an enzymatic DNA polymerase activity, are wherein synthetic Oligodesoxynukleotidverbindungen used as primers, represented by the formula are writable, said SeqA, SEQB and SeqC for sequences of naturally occurring nucleotide building blocks or their synthetic analogs are provided, n a natural number from 0 to 3, representing SeqA, SEQB and SeqC oligonucleotide N1 a at one to six-membered ring of the pyrimidine or the five-membered ring is purine bases constituent atom modified Phosphoribonukleotidrest or a nucleotide analogue and R1 and R2 N1 covalently linked, identical or different molecular radicals comprising twelve and heavier, representing at least ten atoms of atomic weight, as well as reaction mixtures for performing the method.