Green synthesis, characterization, cytotoxicity, antioxidant, and anti-human ovarian cancer activities of Curcumae kwangsiensis leaf aqueous extract green-synthesized gold nanoparticles

Abstract The present work indicated the green synthesis, characterization, cytotoxicity, antioxidant, and anti-human ovarian cancer activities of gold nanoparticles containing Curcumae Kwangsiensis Folium (Leaf) aqueous extract. Characterization of gold nanoparticles was done by Field Emission Scanning Electron Microscopy (FE‐SEM), Transmission Electron Microscopy (TEM), Fourier Transformed Infrared Spectroscopy (FT‐IR), and UV–Visible Spectroscopy (UV-Vis). MTT assay was used on common ovarian cancer cell lines i.e., SW-626, PA-1, and SK-OV-3 to survey the cytotoxicity and anti-ovarian cancer effects of gold chloride, Curcumae Kwangsiensis Folium leaf aqueous extract, and gold nanoparticles. The best results of cytotoxicity and anti-ovarian cancer properties were seen in the case of gold nanoparticles. They had very low cell viability and high anti-ovarian cancer activities dose-dependently against PA-1, SW-626, and SK-OV-3 cell lines without any cytotoxicity on the normal cell line (HUVEC). For investigating the antioxidant properties of gold chloride, Curcumae Kwangsiensis Folium leaf aqueous extract, and gold nanoparticles, the DPPH test was used in the presence of butylated hydroxytoluene as the positive control. The best results of antioxidant properties were seen in the case of gold nanoparticles. They inhibited half of the DPPH molecules in the concentration of 153 µg/mL. Maybe significant anti-human ovarian cancer potentials of gold nanoparticles synthesized by Curcumae Kwangsiensis Folium leaf aqueous extract against common human ovarian cancer cell lines are linked to their antioxidant activities.