Non-toxic cell refrigerating liquid containing cholesterol and preparation method thereof

The invention discloses a non-toxic cell refrigerating liquid containing cholesterol. A preparation method of the non-toxic cell refrigerating liquid comprises the following steps of: dissolving self-made CLC (Cholesterol-Loaded Cyclodextrin) and EDTA (Ethylene Diamine Tetraacetic Acid) in m199 cell culture liquid, stirring and uniformly mixing the liquid, and filtering the liquid to remove bacteria through a filter membrane to obtain the non-toxic cell refrigerating liquid. The contract that cholesterol can not be dissolved in the culture liquid is solved, and the action of protection cell membranes is exerted.