Gene Transfer to a Animal Cells in Culture by Microinjection: A Novel Tool to Create Recombinant Cell Lines?

We are using microinjection of plasmid DNA into the cytoplasm and nucleus of Chinese hamster ovary (CHO) cells to better understand the mechanisms involved in DNA degradation and integration within the cell and to identify the requirements for the establishment of highly productive cell lines. Microinjection is an excellent tool for these studies since it allowed us to control the timing and the location of DNA transferred into single cells.