Synthesis of Organothiophosphate Antigens for the Development of Specific Immunoassays

By use of t-butyl 3-[ chloro ( methoxy ) phosphorothioylamino ] propanoate (1), a general method was developed that allowed the preparation of analogues of specific organothiophosphates ; these analogues are suitable for use as haptens in the development of tests for detection of the individual organophosphates. The bifunctional reagent (1) was conveniently prepared from readily available starting materials.