Analysis of In-vivo Cytotoxicity and Irritability of an Epoxy Nanocomposite

Objectives: To assess a high-performance epoxy nanocomposite through the analysis of in vivo accumulated irritability on the oral mucosa, and cell viability through in vitro cytotoxicity/biological reactivity in mouse cells. Materials and Methods: Nanocomposite samples were manufactured in epoxy resin Araldite GY 260 using the hardener Aradur HY 951, a diluent, and carbon nanotubes previously dosed as reinforcement. The samples were then used in the tests. Results: Cell viability test showed cellular growth above 70%, and an irritability index of 0.00, which is considered ideal for materials used with humans. Conclusion: The sample was considered non-irritating to the oral mucosa and non-cytotoxic, rendering its use viable in dental prostheses.