Apoptosis effect of HepG2 cell induced by silybin-phosphatidylcholine compound

Objective To investigate the apoptosis effect of Silybin-phosphatidylcholine Compound(SPC) on human hepatoma cell HepG2 and its mechanisms.Methods HepG2 cells were treated with differetent concentrations of SPC.MTT assay was used to observe the inhibitory rate of SPC,and fluoreacence microscope,flow cytometry and laser conlocal microscopy were used to observe the influence of SPC on cell apoptosis of HepG2.Results The proliferation of HepG2 was inhibited by different concentration of SPC and the GI50 is 46.8μg/ml;After treating with 50 μg/ml of SPC for 24 h,morphological changes of early apoptosis were observed.At the dosage of 75 μg/ml and 100 μg/ml,SPC could promote the apoptosis of HepG2 cell and the apoptotic rates are(17.22±3.45)% and(25.50±5.72)% respeatively.After treating with 50 μg/ml,75 μg/ml,100 μg/m of SPC for 24 h,the intracellular Ca2+:level was remarkably increased(P0.05,P0.01).Conclusions SPC can induce cell apoptosis of HepG2 and the mechanism is related with the increase of intracellular Ca2+.