Dot immuno-gold filtration assay (DIGFA) quick detection card for leather hydrolyzate in milk and preparation method thereof

The invention relates to a dot immuno-gold filtration assay (DIGFA) quick detection card for leather hydrolyzate in milk and a preparation method thereof. The detection card is composed of a sample layer, a colloidal gold labeled layer, a detection layer, a water absorption layer, a plastic lining plate and a plastic cover plate. The preparation method comprises the following steps: preparing a colloidal gold labeled leather hydrolyzed protein solution and a colloidal gold labeled layer; preparing a leather hydrolyzed protein multi-clone antibody solution and detection lines on the detection layer; and assembling the detection card. The detection card has the advantages of simple operation and accurate detection result, can be interpreted by naked eyes, does not need complex operation, special equipment and the like, is suitable for on-site casual detection of leather hydrolyzate in milk for milk product enterprises, and inspection and sanitary departments, and has wide application prospects.