Vigilin interacts with CCCTC-binding factor (CTCF) and is involved in CTCF-dependent regulation of the imprinted genes Igf2 and H19.

CCCTC-binding factor (CTCF), a highly conserved zinc finger protein, is a master organizer of genome spatial organization and has multiple functions in gene regulation. Mounting evidence indicates that CTCF regulates the imprinted genes Igf2 and H19 by organizing chromatin at the Igf2/H19 locus, although the mechanism by which CTCF carries out this function is not fully understood. By yeast two-hybrid screening, we identified vigilin, a multi-KH-domain protein, as a new partner of CTCF. Subsequent coimmunoprecipitation and glutathione S-transferase pulldown experiments confirmed that vigilin interacts with CTCF. Moreover, vigilin is present at several known CTCF target sites, such as the promoter regions of c-myc and BRCA1, the locus control region of β-globin, and several regions within the Igf2/H19 locus. In vivo depletion of vigilin did not affect CTCF binding; however, knockdown of CTCF reduced vigilin binding to the H19 imprinting control region. Furthermore, ectopic expression of vigilin significantly downregulated Igf2 and upregulated H19, whereas depletion of vigilin upregulated Igf2 and downregulated H19, in HepG2, CNE1 and HeLa cells. These results reveal the functional relevance of vigilin and CTCF, and show that the CTCF–vigilin complex contributes to regulation of Igf2/H19. Structured digital abstract CTCF physically interacts with Vigilin by two hybrid (View interaction) CTCF physically interacts with L31 by two hybrid (View interaction) CTCF physically interacts with CHD1L by two hybrid (View interaction) CTCF physically interacts with Ral A by two hybrid (View interaction) CTCF physically interacts with DDX17 by two hybrid (View interaction) CTCF physically interacts with ND4 by two hybrid (View interaction) CTCF physically interacts with Vigilin by pull down (View interaction) CTCF physically interacts with Vigilin by anti bait coimmunoprecipitation (1, 2)