High-resolution Raman scattering in oligonucleotides
2014
The high-resolution spectra of short single-stranded oligonucleotides have been measured by the high-sensitivity nonresonant Raman scattering method using the example of the system d(20G, 20T), where G is guanine and T is thymine. The samples under study have been prepared by the chemical synthesis using the solid-phase amidophosphite method on an automatic synthesizer. It has been shown that the spectra observed in the frequency range of 500–1500 cm−1 can be sufficient for the resolution of spectral components corresponding to vibrations of individual oligonucleotide molecules, which contain a large number of atoms in the unit cell.
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