Detection of influenza virus using a lateral flow immunoassay for amplified DNA by a microfluidic RT-PCR chip

Influenza virus RNA was amplified by a continuous-flow polydimethylsiloxane microfluidic RT-PCR chip within 15–20 min. The amplified influenza virus RNA was observed with the naked eye, as the red color at the test line, using a lateral flow immunoassay within 1 min.