The effects of cell transplantation after statins medicine intervention on the infarcted myocardium

Objective To study the effects of mesenchymal stem cells(MSCs)transplantation besides of statins on repair of the infarcted myocardium and the possibility of its therapy for acute myocardial infarct(AMI).Methods MSCs of rats were removed and cultured in vitro.The acute myocardial infarction(AMI)model was established through the ligation of left anterior descending coronary artery.The survival rats were randomly divided into 4 groups:the combination of MSCs transplantation and atrovastatin ( group A),atrovastatin group(group B),MSCs transplantation group(group C),and control group(group D).Bats in group A and B were administrated orally with 10mg/Kg.d atrovastatin solution during 10 days after operation;rats in group C and D were fed normally.10 days later,MSCs were injected into 5 points in myocardial infarct region at the concentration of 2×106 cells/50μl/point respectively in group A and C;PBS was injected at the same place and dose in group B and D.3 weeks later,the left ventricular ejection fraction(LVEF),left ventrleular systole pressure(LVSP),left ventricular end diastole pressure (LVEDP),and the maximal rate of left ventricular pressure(+dp/dtmax)were examined respectively.The morphological changes of myocardium at cellular and histological level were measured immunohistochemially.Results Compared with group B and D,the mobility of anterior wall of left ventricle got stronger in group A and C,and the LVEF,LVSP,±dp/dtmax all increased(P＜0.05),while the LVEDP decreased(P＜0.05).Those changes were more significant in group A than in group C(P＜0.05).The 5-bromodeoxyuridine(BrdU)positive cells could be found in infarcted myocardium,and showed positive expression oftroponin(TnT),connexin43(Cx-43).Conclusion Treatment with the combination of MSCs and statins for acute myocardial infarction could obviously promote cardiac function. Key words: Acute myocardial infarction;  Statins;  Mesenehymal stem cells;  Inflammation reaction