Characterization of Dasypyrum Villosum (L.) Candargy Chromosomes by Fluorescent in situ Hybridization

Fluorescent in situ hybridization (FISH) was utilized to study disomic wheat (Triticum aestivum)/Dasypyrum villosum chromosome addition lines to characterize the V genome chromosomes of D. villosum L. Candargy. The V genome specific DNA sequence, pHv62; the rye sequence, pSc119.2, the 18S-5.8S-26S rDNA genes, pTa71, and the 5S rDNA sequence, cP5S, were used as probes. Six of the seven added D. villosum chromosomes hybridized with pHv62. One of these, 3V, was shown to be a Robertsonian translocation with a wheat chromosome. The presence of the seventh addition line chromosome was verified by FISH using total genomic D. villosum DNA as probe. FISH with cP5S detected a site on 5V, whilst pTa71 revealed a single site on the short arm of IV, but pSc119.2 detected sites on all the chromosomes of D. villosum. The pHv62 probe was also used in the application of FISH to a number of other species in the Triticeae, including five species of Aegilops, barley and the tetraploid Dasypyrum breviaristatum. Sites were detected only in Thinopyrum bessarabicum and Secale cereale, and this together with the above evidence for pTa71 and pSc119.2 suggests that D. villosum is phylogenetically closest to Th. bessarabicum and S. cereale, and also is not related to Dasypyrum breviaristatum.