Detection method and surface plasmon resonance detection system

SPR detection method, comprising the steps of: providing a SPR sensor comprising a sensor surface that supports SPR; putting a sample to be analyzed in contact with said surface sensor and monitoring at least a resonance condition in said sensor surface that supports SPR illuminating said sensor surface with a light beam test excite SPR and detecting the beam light reflected or transmitted test; determining at least one property of said light beam reflected or transmitted test; It characterized by comprising illuminating said sensor surface with a beam of reference light conditions selected to not excite SPR on said sensor surface and detecting the intensity of the light beam reflected or transmitted reference; wherein determining the at least one property of said reflected light beam or transmitted test takes into account the detected intensity of the light beam reflected or transmitted reference.