The direct separation of the diastereoisomers and enantiomers of the fungicide triadimenol by micellar electrokinetic capillary chromatography.

: The direct chiral separation of the four enantiomers of the systemic fungicide triadimenol has been investigated using micellar electrokinetic capillary chromatography (MECC) with cyclodextrin (CD) chiral selectors and organic modifiers. The capillary operating temperature, applied voltage, and column diameter were all shown to be important factors in achieving an enantioselective separation. The addition of various CDs to the micellar buffer mixture was evaluated. The type of CD and its concentration affected the degree of separation, with the best separation of the enantiomers demonstrated for heptakis 2,6-di-O-methyl-beta-cyclodextrin (di-OMe-beta-CD). The addition of organic modifiers and CDs to the background electrolyte further enhanced separation. The best separation of the four enantiomers was obtained using an MECC buffer system consisting of 20% methanol, 0.02 M hydroxypropyl-beta-cyclodextrin (HO-Pr-beta-CD), and 0.05 M sodium dodecyl sulfate (SDS) in 0.02 M sodium borate and 0.02 M disodium hydrogen phosphate. The reported method demonstrates how the separation of enantiomers can be achieved by systematically varying the parameters in an MECC buffer system.