ENDOMETRIAL CYTOLOGY IN DIFFERENT PATHOLOGICAL CONDITIONS OF UTERUS IN SHE-BUFFALOES

Endometrial cytological studies in she-buffaloes were scanty. Hence, a study was conducted to assess the status of the uterus of she-buffaloes using endometrial cytology. Uterine endometrial smears prepared from uterine luminal fl uid by gently touching the uterine surface of 54 reproductive tracts collected from a slaughterhouse and four uterine biopsy imprints of she-buffaloes were utilized for the study. Uterine endometrial smears and uterine biopsy imprints were stained with Leishman-Giemsa stain. The differential counts for epithelial cells, lymphocyte, neutrophil and monocyte for 23 cases of normal endometrium were 99.73 (97-100), 0.26 (1-3), 0 and 0 percent respectively. In 29 cases of subacute nonsuppurative endometritis, the differential counts were 80.45 (44-100), 4.97 (0-45), 4.03 (1-54) and 0 percent for epithelial cell, lymphocyte, neutrophil and monocyte, respectively. In two cases of subacute non-suppurative metritis, the differential counts were 99 (98-100), 1 (0-2), 0 and 0 percent for epithelial cell, lymphocyte, neutrophil and monocyte respectively. In four acute suppurative endometritis cases from biopsy, the differential counts were 50.25 (19-100), 8 (0-29), 39.75 (0-52) and 0.5 (0-1) for epithelial cell, lymphocyte, neutrophil and monocyte, respectively. The average neutrophil count in endometrial cytology was higher in endometritis cases. Hence, endometrial cytology in she-buffaloes provides simple, quick and useful information about the endometrial status in she-buffaloes.