Identification method for blood serum proteome of broiler chickens

The invention provides an identification method for blood serum proteome of broiler chickens. The identification method comprises the following steps: (1) collecting a blood serum sample; (2) carrying out SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis); (3) carrying out in-gel enzymolysis on protein gel; (4) analyzing protein by nano-HPLC-MS/MS (nano-High Performance Liquid Chromatography-Mass Spectrometry/Mass Spectrometry); searching a protein data base through Protein Pilot software to identify obtained data. By adopting the method provided by the invention, the quantity and types of blood serum protein expression of the broiler chickens can be rapidly found at high throughput, and a method and a concept are provided for development of research on nutrients of the broiler chickens and immune functions of the broiler chickens.